FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851235

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851235
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54000849
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA717770.13291828059962538No Hit
CGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTG696890.12905167472459553No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG605410.11211120032575785No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG176850.013.4521845
TGCCCGT278650.012.29153444
GGCATAG190600.012.14991935
GGGAGGT295850.011.8592233
TAGCGTA10700.011.7758237
ATAGGGG205050.011.77371638
GTTGTCG31650.011.73793326
TCAGACG144550.011.3969222
GGTCACT291550.011.1990337
AGGGATC164450.011.1810552
ATAGCTC75750.011.167788539
GAGCTTA215600.011.09834244
ACCCGGC85900.010.8683015
GCCCGTG321100.010.82632945
TGGCATA215400.010.82463134
GTCACTC307850.010.7801958
GGAGGTC325200.010.6964744
GCGTCGA46400.010.32108511
ACTCTCC326800.010.31295911
CGTCGAT45050.010.28141812
Produced by FastQC (version 0.11.5)