FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851236

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851236
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50113385
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT6690061.3349846552971827No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG1504330.30018526986353045No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1442570.28786121711794166No Hit
CCACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAAC877250.1750530322387921No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT947600.033.3179845
AGAACGA1114200.028.3805644
ACCGCGT1222650.026.0070366
AGACCGC1225700.025.9902044
GACCGCG1230300.025.8582045
CAGACCG1233650.025.8154183
CCGCGTT1239100.025.6654017
TCAGACC1250700.025.617862
CGCGTTC1243750.025.5741128
GCGTTCT1285950.024.7727879
AAGAACG1280800.024.65394843
CTCAGAC1329850.024.5555381
ATACGGA1288750.024.4563533
AATACGG1288950.024.45080832
TACGGAG1295050.024.26276634
CAATACG1316650.023.9603331
ACGGAGA1325350.023.76435735
CGTTCTC1351200.023.72136710
CCCAATA1342250.023.55821829
CCCCAAT1357100.023.3216428
Produced by FastQC (version 0.11.5)