FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53130503
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG625510.11773086356814653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG196950.015.52078245
GGCATAG201250.015.39328135
ATAGGGG207200.015.31790138
GGTCACT309050.014.7892047
GTCACTC322850.014.2543948
GGGAGGT352900.014.0545143
AGGTCAC335850.013.9982936
GGAGGTC340000.013.8116584
GAGGTCA353450.013.4765425
GTTGTCG32850.013.42536726
GAGCTTA232100.013.37377744
TGGCATA235700.013.2787134
ACTCTCC354700.013.27674111
CATAGGG236200.013.15156537
TCACTCT376500.012.4079549
TAGGGGA365950.012.3793839
GCATAGG254000.012.29373836
CACTCTC396800.011.69288410
CGTAGGG123500.011.58387337
GCGTAGG125450.011.35170936
Produced by FastQC (version 0.11.5)