FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851240

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851240
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41151040
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG18700.021.7775756
CGAACGA64000.018.38622932
TCGACGA24050.017.7750597
GTCCGTT29550.017.4559571
ACGAACG70250.016.97463631
ACGCGTC11650.016.80208640
GCCGGCT135850.016.59741
CCGGCTT167300.016.1382032
CCCCCCT37600.015.9352721
TCACGCT163450.015.7200438
CGACGAG26050.015.7194088
CTCCGTT69650.015.6527231
CCCGTAT28400.015.5454541
TTCACGC168850.015.40385157
CGGTATA31500.015.35671620
CTCCGAT39400.014.9785821
CTCCGGT43400.014.7917931
AGTGCTA74850.014.78936612
AAGCGCG13300.014.71763518
AACGAAC82000.014.59717230
Produced by FastQC (version 0.11.5)