FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51204554
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG604180.11799341128915994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA155500.021.39794323
ACGCTAC156550.021.31240824
CGCTACC163100.020.42912525
TTCGACG20550.018.937546
AAAACGC180050.018.59193221
TCACGCT193500.018.0775158
GAAAACG188200.017.7508820
ACGCTCA210300.016.91149110
AAACGCT198500.016.89857722
TTCACGC209650.016.8673537
GTCCGTT36300.016.3747391
CCGGCTT224400.016.369942
GCCGGCT215650.015.6922991
CACGCTC231800.015.0711839
TCGTGGT224550.014.99748440
TCGGCGC74900.014.95710645
GTTTTCG226600.014.75195245
CTACACG49250.014.72131145
CGGCTTC266600.014.5892633
CGTGGTT233000.014.56944441
Produced by FastQC (version 0.11.5)