FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47187833
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG710860.15064476472144842No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT645630.13682128611415573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG142850.023.2358765
ACCGCGT145250.022.8446796
AGACCGC144700.022.8299644
CCGCGTT158350.020.968617
TTCGACG27700.020.70956
CAGACCG162300.020.5345063
CGCGTTC167650.019.7920468
CGGTATA40100.019.46616720
GCCGGCT225550.018.2661271
CCGGCTT259950.017.4699252
TCACGCT253650.017.1878368
TTCACGC256150.017.1166527
GTTCGAC33000.017.0479955
TCAGACC200400.017.0464422
TCGACGA33750.016.8635147
GAACGAT195600.016.84855845
CGACGAG33850.016.8137328
ACGAACG41400.016.36486231
ACGCTAC259850.016.05302824
AACGCTA262050.015.94313523
Produced by FastQC (version 0.11.5)