FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851245

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851245
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47187833
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA516340.10942227416969963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGG164700.013.4497738
GTTGTCG30950.012.8838426
GTCACTC228900.012.8455278
GGCATAG169600.012.729671535
AGCTTAG171550.012.63852445
GGGAGGT247000.012.5756453
GGAGGTC238700.012.5512964
GGTCACT234450.012.4067887
ACTCTCC255950.011.48875611
GAGCTTA202300.010.96168444
GAGGTCA282200.010.8641555
CATAGGG200600.010.81858637
AGGTCAC283650.010.7529716
ATATAGC44350.010.7521126
TGGCATA207050.010.64443634
GCATAGG212850.010.2910136
TCACTCT292650.010.2167099
AGGGATC133750.09.9613862
CACTCTC292450.09.93180410
TGTCGTT39700.09.70372128
Produced by FastQC (version 0.11.5)