FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851246

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851246
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53033473
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG37850.024.9612336
TCGACGA46050.021.1511677
CGGTATA59350.019.74661620
CGACGAG47500.019.6530788
AACGCTA171200.018.197923
ACGCTAC172100.018.18115824
GTTCGAC55050.017.2031925
CGCTACC182200.017.18565825
ACCGCGT101000.016.9713446
GACCGCG101850.016.8518585
TCACGCT199200.016.4191688
AAAACGC193750.016.28889521
AGACCGC104900.016.2760774
GCCGGCT205200.016.108811
CCGCGTT110200.015.5338527
GAAAACG203100.015.48363420
GTCCGTT42950.015.2981431
CGCGTTC114700.014.9244168
ACGCTCA214100.014.88775610
CCGGCTT230900.014.8569972
Produced by FastQC (version 0.11.5)