FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851250

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851250
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51758413
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT740700.1431071698430939No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG730460.14112874751395488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT170250.022.835616
GACCGCG173600.022.3433865
AGACCGC175800.022.217354
TTCGACG23600.021.449966
CAGACCG186050.021.1384533
CCGCGTT187150.020.7494477
CGCGTTC192850.020.1244968
TCAGACC235650.017.7012582
GAACGAT228200.017.3454845
AACGCTA248000.016.80259323
ACGCTAC248350.016.77891324
TCACGCT270250.016.250618
TCGACGA31300.016.1012367
CTCAGAC294150.016.0282361
GCGTTCT245100.015.9812599
CGCTACC260850.015.97486425
AATACGG246600.015.95868832
ATACGGA247600.015.86697233
TACGGAG244400.015.83408134
GCCGGCT259050.015.7067151
Produced by FastQC (version 0.11.5)