FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851251

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851251
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51758413
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG36650.014.1183226
AGCTTAG168350.012.6821745
GGCATAG175500.012.07574335
GTCACTC224300.011.9480218
ATAGGGG185950.011.67529438
GGTCACT233600.011.3765647
CATAGGG187350.011.37195437
ACCCGGC84450.011.2739675
ACTCTCC239400.011.22252811
TGTCGTT46050.011.18752928
GGAGGTC250850.011.1354024
GGGAGGT275450.011.040753
AGGTCAC252050.010.9466
GCATAGG194400.010.83224336
GAGCTTA199650.010.64879444
TGGCATA206300.010.37110734
TATAGCT61300.010.2785437
GTCGTTC51150.010.11604929
AGGGATC142800.010.0422342
ATATAGC61400.010.0056656
Produced by FastQC (version 0.11.5)