FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851252

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851252
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49400734
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT757640.15336614229254164No Hit
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT522940.10585672674418158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT155350.024.677186
AGACCGC156400.024.5546634
GACCGCG156250.024.535045
CAGACCG164150.023.6420673
CCGCGTT164800.023.2211827
GAACGAT171500.022.05364445
CGCGTTC176900.021.620138
AATACGG195100.019.49166132
TCAGACC204250.019.4410552
ATACGGA198850.019.14671133
AGAACGA201100.018.93064944
TACGGAG204400.018.54976734
GTTAGTT146000.017.96895844
CAATACG212750.017.94866431
AAGAACG220800.017.28238143
GCGTTCT224600.017.1286689
CTCAGAC288500.016.4079931
CCCAATA238700.016.14843829
ACGGAGA236650.016.12643435
CCGTACG125500.015.8469524
Produced by FastQC (version 0.11.5)