FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49400734
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG534300.10815628771831609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG158400.016.20218545
GGCATAG164600.015.19249835
ATAGGGG172250.014.63503138
GAGCTTA184800.013.94821444
GTCACTC265950.013.6561058
GGTCACT270550.013.3301567
TGGCATA193000.013.21335734
TCAGACG102800.013.1301812
CATAGGG193850.013.05074637
GGGAGGT304200.012.8094243
AGGTCAC291150.012.7632946
GCATAGG204650.012.32872436
GAGGTCA308800.012.1840175
ACTCTCC313500.011.75691711
TAGCGTA8450.011.72008637
GGAGGTC326200.011.6563434
GTTGTCG30500.011.36183826
TGCCCGT144200.010.850121544
CGTCGAT30400.010.7349112
TCACTCT353150.010.6275849
Produced by FastQC (version 0.11.5)