FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851254

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851254
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51944666
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1342650.25847697239982254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT271650.025.13079545
AGACCGC308250.023.7803884
ACCGCGT315250.023.1667376
GACCGCG316750.023.0925335
CAGACCG327400.022.4856283
CCGCGTT327000.022.3342917
CGCGTTC329200.022.1782058
AGAACGA322000.021.25349244
TCAGACC370300.020.2329222
AATACGG365700.019.80956632
GCGTTCT371500.019.7376219
ATACGGA368000.019.65801833
TACGGAG369000.019.54574434
TTCGACG18150.018.8366936
CAATACG388700.018.74540531
AAGAACG365350.018.70842643
ACGGAGA396800.018.23551435
CTCAGAC457150.018.0917551
CCCAATA424000.017.28914329
CGGAGAG418250.017.27207636
Produced by FastQC (version 0.11.5)