FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51944666
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG31500.014.07290126
AGCTTAG177450.013.34104645
GGGAGGT296600.012.1463093
GGCATAG194400.012.1410835
GTCACTC274550.011.8547748
GGTCACT273400.011.8224817
ACCCGGC84800.011.8039325
ATAGGGG210650.011.42763738
GAGGTCA308150.011.1611215
ATAGCTC84400.011.06266839
ATATAGC46900.011.0308556
ACTCTCC295800.010.9818611
GGAGGTC305150.010.8727854
TGGCATA223250.010.79393734
AGGTCAC316150.010.7575356
GAGCTTA220300.010.74397844
TGTCGTT43200.010.52175128
TCAGACG109150.010.3695042
CATAGGG232600.010.32107437
TCACCCG101750.010.2835423
Produced by FastQC (version 0.11.5)