FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55263145
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1758930.3182826456945221No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG734160.1328480309978739No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG577800.10455431011029141No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA558240.10101488071299598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG453400.020.5151215
AGACCGC460950.020.476934
ACCGCGT458100.020.422766
CAGACCG468450.020.2260323
CCGCGTT477900.019.5620177
CGCGTTC481600.019.392768
TCAGACC521800.018.772342
GCGTTCT516150.018.0944849
CTCAGAC572050.018.0760821
CGTTCTC559450.017.20081310
GAACGAT539150.017.10171345
TTCGACG17200.016.6136686
CCGGCTT303750.016.268592
TTCACGC302950.016.123847
TCGTTAC26100.015.08605344
TCACGCT325550.014.9766468
AACGCTA315100.014.95922223
ACGCTAC316150.014.91662724
GTCCGCT44400.014.9010581
GCCGGCT300050.014.8124261
Produced by FastQC (version 0.11.5)