FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851258

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851258
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51116923
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG97550.022.7645535
ACCGCGT97400.022.7534146
TTCGACG26850.022.2898226
AGACCGC106850.021.0990314
CCGCGTT105650.020.9340527
CGCGTTC110300.020.1331128
CAGACCG120000.019.0119193
TCGACGA33950.018.2910487
GAACGAT129800.017.07382445
CGACGAG35350.016.8665318
CGGTATA44250.016.82999620
AATACGG139250.015.67291132
CCCGTAT32000.015.4181171
ATACGGA143600.015.21380733
TACGGAG142600.015.16271434
TCAGACC160600.014.9481742
ACGCTAC111600.014.73745324
AACGCTA111650.014.63009623
AGAACGA155550.014.4643644
CGTACGC58100.014.4444835
Produced by FastQC (version 0.11.5)