FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851259

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851259
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51116923
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG111700.013.39566235
AGCTTAG124300.012.20084845
ATAGGGG126750.012.19617538
CATAGGG130850.011.62480537
GCATAGG133200.011.30136236
ACCCGGC76050.011.2131725
GTCACTC184700.010.8656328
TCAGACG66550.010.7196462
GGTCACT186750.010.4934747
GGAGGTC201500.010.4424
GGGAGGT226150.010.3986933
TGGCATA151750.010.31991934
GAGCTTA149200.010.25531544
TCACCCG93600.09.7613353
GTTGTCG28850.09.74786326
ACTCTCC214800.09.48978811
AGGTCAC218700.09.454526
TCGCGCT33150.09.43479645
ACGGACA84850.09.41318418
CGGCCCG93950.09.1958428
Produced by FastQC (version 0.11.5)