FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851260

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851260
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41644791
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT592640.14230831414185752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC128250.022.8417844
CAGACCG135550.021.7776323
ACCGCGT136900.021.2463766
GACCGCG140100.020.7157085
GAACGAT146050.019.96114345
CCGCGTT149000.019.535847
TTCGACG18150.019.2107756
CGCGTTC159900.018.26048
ATACGGA162900.017.83882333
AATACGG163300.017.8089132
TACGGAG162000.017.78508634
AGAACGA169700.017.24556544
TCAGACC180200.017.043322
CTCAGAC233350.016.3088471
GCGTTCT184450.015.9153029
ACGTTCG24250.015.5843026
AACGCTA146950.015.49399123
ACGCTAC148700.015.3880224
ACGGAGA190700.015.31943635
CGGTATA29950.015.02214120
Produced by FastQC (version 0.11.5)