FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41644791
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG108800.015.837553
ACCCGGC105950.015.8198765
AGCTTAG121100.014.56850245
CGGACAC116600.014.42091413
CGGACAG115850.014.19998519
CCGGACA122400.014.01241112
ACGGACA119550.013.91290818
ATAGCTC116050.013.84401539
CACCCGG124050.013.7644974
GACACGG119200.013.709615
GGCATAG131600.013.488535
ACACGGA121550.013.44365216
GTCACTC181850.013.2295988
TAGCTCT126600.012.70811940
CACGGAC129900.012.63118417
GAGCTTA141850.012.53252744
CATAGGG144100.012.47457737
GGTCACT193550.012.3823367
ACTCTCC201950.012.17062411
TGATAGC136800.012.16994737
Produced by FastQC (version 0.11.5)