FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851262

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851262
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51625683
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG532250.10309790961990761No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA169550.023.98008323
ACGCTAC172150.023.68362624
CGCTACC178950.022.77119825
TCACGCT192300.022.0077388
AAAACGC189300.021.66715821
ACGCTCA217700.019.97741710
TTCACGC214350.019.953757
GAAAACG207200.019.78414720
AAACGCT211650.019.3472422
CACGCTC229650.018.4186299
TTCGACG21800.017.54526
GTTTTCG245750.016.5440245
CCGGCTT208150.016.2354722
TCGTGGT251200.016.18370240
GCCGGCT203150.016.0217651
CTACCTC266450.015.58044227
CGTGGTT263050.015.49741641
CCCGTAT30150.015.3259191
TACCTCT280350.014.92038628
CCTCGTG281450.014.70807938
Produced by FastQC (version 0.11.5)