FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851264

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851264
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50238177
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG846370.16847147936916582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGGCT244700.020.2279281
TCACGCT260450.019.8675298
CCGGCTT286650.019.2625792
TTCACGC271300.019.1724857
AACGCTA270050.018.8638423
ACGCTCA282950.018.51030710
ACGCTAC248600.018.428124
CGCTACC253000.017.94752525
AAAACGC289000.017.74238221
CACGCTC300600.017.3411319
GAAAACG299950.017.03453420
AAACGCT306550.016.74909822
CGGCTTC342650.016.6266523
CCCGTAT43900.016.5689771
ACCGCGT50100.016.4804366
GTCCGTT38750.016.3883441
CGTGGTT306450.016.30592241
CCCGGTT55400.016.2188781
TCGTGGT317050.015.74678740
AGACCGC54050.015.36072354
Produced by FastQC (version 0.11.5)