FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851267

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851267
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49906759
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG617230.12367663466185011No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG190050.016.18233935
AGCTTAG194300.016.14912245
GGTCACT281100.015.2989927
GTCACTC284000.015.174338
ATAGGGG208650.014.99586438
GAGCTTA212600.014.72731244
CATAGGG212200.014.62843237
TGGCATA214650.014.43420734
AGGTCAC315800.013.97462756
ACTCTCC310050.013.96950511
GCATAGG225350.013.77708836
GAGGTCA328350.013.7041055
GGGAGGT347700.013.66855053
GGAGGTC330100.013.5297644
TCACTCT361200.012.198539
CACTCTC356900.012.16778410
ACCCGGC89950.011.9118915
TAGGGGA359250.011.90900839
GCTCTGT384100.011.508780522
AGGGGAG378550.011.40279940
Produced by FastQC (version 0.11.5)