FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851268

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851268
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50650200
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG86350.019.2294355
ACCGCGT86700.019.2036726
AGACCGC90200.018.3587784
CCGCGTT102450.016.1415847
CAGACCG104350.016.0202333
GTCCGTT47450.015.4243841
CGCGTTC107200.015.4053678
GCCGGCT153400.014.9592391
TCACGCT164350.014.6482188
CCGGCTT181450.014.5945922
CCCGTAT37700.014.1568831
ATAAACG95700.013.75354821
TTCACGC175650.013.7058657
TAAACGC95850.013.520761522
CTCCGGT65500.013.511761
TTCGACG16000.013.4996626
ACGCTCA184950.013.0166810
CTCCGTT67950.012.9582971
CCCGGAT107600.012.5992561
CCCGGTT53350.012.4522621
Produced by FastQC (version 0.11.5)