FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851270

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851270
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52773542
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG679470.12875201744086082No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGCTA149650.023.99537523
ACGCTAC150700.023.72367724
CGCTACC153150.023.22663325
AAAACGC161350.022.2135621
AAACGCT175100.020.50775522
GAAAACG174850.020.47273820
TCACGCT194950.019.089028
GCCGGCT202950.018.5927161
ACGCTCA200300.018.45615210
CCGGCTT221950.018.1768042
GTCCGTT45550.017.645651
TTCACGC214800.017.5973137
CCCGTAT36600.016.7319181
CACGCTC223300.016.6453579
GTCGGTT48700.016.50431
CTCGGTT120150.015.9651761
TCGTGGT231200.015.65817940
CGGCTTC279950.015.4156033
GTCCGAT31050.015.2270411
GTTTTCG238900.015.03100945
Produced by FastQC (version 0.11.5)