FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851271

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851271
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52773542
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATAG126050.012.34935635
ATAGGGG130200.012.2321338
CATAGGG133100.011.86421237
AGCTTAG137650.011.6354545
GCATAGG139650.011.30774536
GAGCTTA157950.010.154284544
TGGCATA166050.09.75381934
GTCACTC186750.09.701648
GGGAGGT206400.09.5512243
TAGCGTA9200.09.53578837
TCAGACG92100.09.3095682
ACTCTCC200650.09.22025611
GGTCACT194100.09.1719327
TAGGGGA195100.08.9932639
TAATCGG48600.08.8906714
GGAGGTC211300.08.8505674
AGGTCAC220750.08.7375576
TTAACGC47450.08.34369332
ATAATCG57200.08.3013943
ATCGGTT54400.08.2331136
Produced by FastQC (version 0.11.5)