FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851272

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851272
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49523619
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG515360.1040634772672813No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT508160.10260962552029972No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG122250.022.5424675
ACCGCGT122900.022.5329826
AGACCGC123000.022.3509584
AACGCTA132800.021.38098523
CAGACCG131900.021.100563
ACGCTAC134150.020.9812924
CGCTACC139500.020.04768625
TTCGACG24000.019.2156526
AAAACGC154350.018.71535521
CCGCGTT149100.018.4527597
GAAAACG168400.017.09972620
CGCGTTC161200.017.0397458
TCAGACC173850.016.4878752
AAACGCT174950.016.4603922
TCGACGA30500.015.4155477
CGGTATA37350.014.99788520
TCACGCT202500.014.8309428
GTTTTCG192450.014.81243545
CGTGGTT195250.014.76426441
ACGCTCA214550.014.21822610
Produced by FastQC (version 0.11.5)