FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49523619
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG147050.015.73742945
GGCATAG152850.015.17017635
ATAGGGG161800.014.30015238
GTTGTCG28750.013.93531526
CATAGGG169800.013.67991437
GAGCTTA178900.013.137012544
GCATAGG179300.012.993546536
TGGCATA182350.012.70336534
GTCACTC242200.012.4137418
GGTCACT241450.012.3223697
GGGAGGT298900.011.2636463
ACTCTCC263150.011.15207511
GGAGGTC278100.010.9746024
AGGTCAC290400.010.6633886
TAGGGGA278150.010.65131939
TGTCGTT40550.010.45294628
CACTCTC293600.010.04908110
GAGGTCA317650.010.0114145
TCACTCT309450.09.9991049
ACCCGGC86250.09.69335
Produced by FastQC (version 0.11.5)