FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55703450
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1125730.20209340714085036No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG622880.11182072205581522No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT276750.022.84794845
AGACCGC312550.020.609934
GACCGCG317700.020.2830685
ACCGCGT316750.020.2800246
CAGACCG326500.019.7981953
CCGCGTT328450.019.5438947
CGCGTTC337700.019.0352658
ACGCTAC184700.018.83278524
AACGCTA184600.018.80642323
AGAACGA348750.018.18882644
TTCGACG23550.018.0571786
CGCTACC193700.017.96936225
TCAGACC368750.017.6945342
AATACGG364400.017.36236632
ATACGGA370100.017.1375233
TACGGAG375750.016.81396334
CAATACG381200.016.73293731
GCGTTCT388350.016.6221439
AAAACGC211750.016.46949421
AAGAACG386000.016.42752343
Produced by FastQC (version 0.11.5)