FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47401042
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1698090.35823896023214No Hit
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG637640.13452024957594813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT310800.026.4157345
ACCGCGT335750.024.8066256
AGACCGC337500.024.6578964
CAGACCG349850.023.8517573
CCGCGTT349250.023.8219767
GACCGCG349500.023.7919355
CGCGTTC356050.023.35448
AGAACGA368300.022.38321944
TCAGACC386100.022.0552272
GCGTTCT394200.021.1627569
AATACGG395600.020.66479332
TACGGAG395850.020.57788734
ATACGGA398750.020.51880533
CTCAGAC448650.020.0094621
CAATACG423600.019.49562531
AAGAACG429800.019.2384643
ACGGAGA426050.019.19788735
CGTTCTC456900.018.5343610
CGGAGAG442400.018.45802136
CCCAATA453950.018.3163129