FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851283

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851283
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47401042
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG565320.119263201007269No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG187200.016.11211445
GGCATAG188750.015.832437535
ATAGGGG206050.014.56518738
TGGCATA214950.014.01961834
GAGCTTA219050.013.81062644
CATAGGG220700.013.56785437
GGAGGTC318500.013.4343864
GGTCACT310900.013.3436647
GTCACTC314250.013.3013578
GGGAGGT354300.012.9447133
GCATAGG234600.012.79348436
ACTCTCC332200.012.58928711
ACCCGGC88450.012.2841875
TAGGGGA346100.011.79381339
ACGGACA88550.011.7112518
GAGGTCA378900.011.4704295
TCACCCG97100.011.4440883
CACTCTC368300.011.31269310
ATAGCTC86700.011.16491639
TCACTCT385250.011.1471519
Produced by FastQC (version 0.11.5)