FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851285

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851285
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49494088
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG1568290.31686410708284996No Hit
CCGGGAGGTCACTCTCCCCGGGCTCTGTCCAAGTGGCGTAGGGGAGCATAG635980.12849615493470656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGGT560500.019.5863113
GGAGGTC586050.018.6298264
GAGGTCA590850.018.5576275
ACTCTCC597900.018.04541411
GGTCACT603050.017.8293197
GTCACTC607400.017.8049878
TGGGAGG448100.017.5756232
AGGTCAC628050.017.3600736
AGCTTAG434250.017.30492245
TCACTCT652650.016.718769
ATAGGGG452850.016.66028638
GGCATAG459550.016.44470435
TGGCATA471000.016.09362834
CACTCTC689250.015.77142610
GAGCTTA478150.015.74435944
TCTCCCC709000.015.23468413
GCGTTCG70750.015.04015715
CTCCCCA519800.014.93197214
CTGGGAG589300.014.846771
CGTTCGC72700.014.75981816
Produced by FastQC (version 0.11.5)