FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851287

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851287
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49206578
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG154700.014.37305345
GGCATAG162050.013.96875335
ATAGGGG170500.013.59446538
GGGAGGT265250.012.7838763
GTCACTC239000.012.7585758
CATAGGG179850.012.57491137
GGTCACT242700.012.5362527
ACTCTCC254700.012.14842411
GGAGGTC265150.012.0081154
GAGCTTA190200.011.9117544
GCATAGG191750.011.88757736
ACCCGGC77700.011.64294055
TGGCATA198400.011.54555434
GAGGTCA295950.010.9725025
AGGTCAC293300.010.94121556
TCACTCT287700.010.880489
CACTCTC291250.010.46969810
GCGTAGG74600.010.3460536
TCAGACG97350.010.3318392
GGCGTAG74950.010.32750735
Produced by FastQC (version 0.11.5)