FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851288

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851288
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57599688
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT914840.1588272492031554No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT167500.026.30341745
AACGCTA76900.022.2352323
AGAACGA202050.021.81145944
ACGCTAC78350.021.79501324
AGACCGC203650.021.7845444
ACCGCGT208750.021.2830336
GACCGCG209100.021.2382935
CCGCGTT217450.020.4522087
AATACGG219000.020.25882132
CGCGTTC221250.020.0602688
CAGACCG224550.019.9876123
ATACGGA223900.019.79527933
TACGGAG223600.019.78213934
CGCTACC86800.019.59587925
AAGAACG240050.018.41564643
CAATACG246350.017.99177631
ACGGAGA257300.017.25972435
TCAGACC268450.016.95392
AAAACGC105950.016.28615821
GCGTTCT273900.016.2781319
Produced by FastQC (version 0.11.5)