FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851289

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851289
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57599688
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG723850.12566908348531333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG208150.016.38980545
GGCATAG225050.015.16477635
ATAGGGG237900.014.53499538
GAGCTTA249500.013.90603244
GTTGTCG33800.013.44504426
GTCACTC358400.013.4431848
GGTCACT355400.013.436267
CATAGGG260100.013.19055637
TGGCATA263450.013.02272434
ACTCTCC380200.012.60168811
GGGAGGT406800.012.6000413
GCATAGG275300.012.49484136
AGGTCAC392500.012.4582256
GGAGGTC394750.012.3294524
GAGGTCA411750.012.0775635
ATAGCTC99200.011.20368639
TGTCGTT42500.011.1162828
ACCCGGC106750.010.961155
ATATAGC50300.010.8263866
TCACTCT456700.010.7815579
Produced by FastQC (version 0.11.5)