FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45921500
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA1020860.22230545604999835No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG695710.1514998421218819No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAAC497910.10842633624772711No Hit
GTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA489700.1066385026621517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG32250.013.89985826
ATATAGC38850.013.6061916
AGCTTAG133400.013.31945645
AGGGATC198750.013.1919312
GGCATAG134500.013.1424335
GTTCTAT207300.013.136351
ATAGGGG140200.012.89685838
TCAGACG113050.012.6612372
TTCTATA644300.012.5329672
TGTCGTT36850.012.53148328
AACTCTA88500.012.17327645
TCTATAG659250.012.0552333
GTCACTC235200.011.966188
CAGGGAT238400.011.80028251
CACCGAA674450.011.728374545
GAGCTTA155100.011.70205844
GGGAGGT256200.011.7018123
TATAGCT45550.011.6557377
CGTCGAT37950.011.501863512
CGTGGCG220750.011.3572491
Produced by FastQC (version 0.11.5)