FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851293

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851293
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54454061
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG155250.015.11838345
ATAGGGG162650.014.13763338
GGCATAG168900.013.60653535
GTTGTCG27950.013.3730426
GAGCTTA185550.012.69035244
GGGAGGT292650.012.499943
GGTCACT283150.011.9044227
CATAGGG197150.011.83579437
TGGCATA198600.011.75307234
GTCACTC290700.011.7265278
ACTCTCC302100.011.46249711
GGAGGTC313600.011.2408734
GCATAGG211600.011.04883736
GAGGTCA329650.010.8406475
AGGTCAC325450.010.7791466
TGTCGTT35100.010.58591428
TAGCGTA6650.010.15114637
GCGTTCG24250.09.74094415
TAGGGGA353750.09.68639539
TCACTCT377100.09.4010639
Produced by FastQC (version 0.11.5)