FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851295

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851295
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51712965
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG95750.011.20702845
GGCATAG100800.010.40016935
ATAGGGG104400.010.19231338
TCAGACG94100.010.1624482
GTGCAAG209350.010.05062613
GACCTCC240500.010.0208985
TGTGCAA215450.09.5467212
ACCCGGC73900.09.500415
GAGCTTA114550.09.465919544
ATAACGC65500.09.37632439
GGTTCTT226400.09.31056736
GGACCTC264750.09.2643374
CGTGGCG141550.09.1243051
GTTGTCG19500.09.11512426
CATAGGG114400.09.08508337
AGCGCTC63250.08.9301359
CAGACGT104900.08.8802323
GTGGCGA144200.08.83182052
TCACCCG85500.08.7898173
ACGGACA81750.08.75299918
Produced by FastQC (version 0.11.5)