FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851296

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851296
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52309516
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGACG43550.025.5113096
GTTCGAC50550.021.620515
CGGTATA67750.021.2786420
AACGCTA109200.021.0897423
TCGACGA53450.020.9544167
ACGCTAC110900.020.76645524
CGACGAG54400.020.340438
CGCTACC116200.019.79992125
AAAACGC136200.017.27228421
ATGACGG84350.016.69139916
TCACGCT146700.016.6032188
CCGGCTT175800.015.8083482
CAGTTCG75900.015.7365913
AAACGCT150850.015.57995522
GAAAACG151650.015.48293520
TTCACGC159400.015.2944877
AGTTCGA73250.015.2901314
CCCGTAT32100.015.2404891
TCGTTAC33750.014.92789244
GTATAGA109700.014.43315222
Produced by FastQC (version 0.11.5)