FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851298

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851298
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44377995
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1313860.2960611447182325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT236900.026.22152545
AGACCGC245850.025.5310134
CAGACCG256950.024.535353
GACCGCG261000.024.0567955
ACCGCGT262350.023.9329786
CGCGTTC271300.023.0770828
CCGCGTT273200.022.9659967
AGAACGA279300.022.4264144
AATACGG287900.021.62888732
ATACGGA288500.021.60718533
TCAGACC297300.021.5610942
TACGGAG289250.021.48860534
GCGTTCT304700.020.672989
CAATACG304000.020.53530131
AAGAACG309800.020.25654243
CTCAGAC351150.019.7880461
ACGGAGA318250.019.63620635
TTCGACG19400.019.3656776
CCCAATA332250.019.0193629
CGGAGAG342750.018.2854736
Produced by FastQC (version 0.11.5)