FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851299

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851299
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44377995
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG764930.17236695799348303No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG206650.017.78442445
GGCATAG230450.015.8804235
ATAGGGG235400.015.6865738
GAGCTTA242550.015.25417544
AGGTCAC333900.015.11595256
GTCACTC326200.015.0930538
GGGAGGT344250.015.0222443
GGTCACT340500.014.4529977
TGGCATA255400.014.44339134
ACTCTCC342350.014.341495511
GGAGGTC360100.014.1175284
GAGGTCA365950.013.9777345
CATAGGG268200.013.76872837
GTTGTCG28500.013.74165126
TCACTCT380850.013.0570389
GCATAGG284150.013.02826636
CACTCTC386400.012.78226410
TGGGAGG312200.012.5836062
TAGGGGA416700.011.68408239
TGTCGTT35300.011.56087828
Produced by FastQC (version 0.11.5)