FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54041687
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG125400.012.62432845
GGCATAG126300.012.52968135
ATAGGGG132650.012.19575138
CATAGGG140500.011.2916737
GCATAGG143100.011.13524736
ACCCGGC78400.010.78620155
GGTCACT198750.010.5853827
GTCACTC202700.010.5327288
GAGCTTA154450.010.36761444
TGGCATA158700.010.05681834
GGGAGGT243950.09.7759223
ACTCTCC235450.09.481647511
ATAGCTC82100.09.3727639
AGGTCAC244350.09.30069356
GTTGTCG27100.09.22084726
GGAGGTC258100.08.8988364
TCACCCG103800.08.8433243
GCGTAGG60350.08.61471636
TAGGGGA248200.08.51232739
TAGCTCT91900.08.49500140
Produced by FastQC (version 0.11.5)