FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46091786
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG579040.12562759013070138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG48550.017.44213326
GGAGGTC298000.014.9174434
GGGAGGT314900.014.8377583
AGCTTAG210950.014.55063245
GCTAAGT59050.014.52799114
GGCATAG211900.014.44989435
ATAGGGG217100.014.318439538
TGTCGTT58400.014.30217128
ATATAGC66950.014.2583196
TATAGCT68200.013.963227
GGTCACT314200.013.8318037
GTCACTC317150.013.7803888
AGGTCAC336400.013.2870046
GAGCTTA232000.013.23176944
ACTCTCC332400.013.08055711
TGGCATA239150.012.89921234
TCGTTCC63850.012.86900730
GAGGTCA352850.012.79560855
CAATATA83100.012.5694664
GTCGTTC69000.012.07234829
Produced by FastQC (version 0.11.5)