FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53408462
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG603300.11295962800801117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGT64100.022.34308445
ACGTTCG38000.020.6604966
TTCGGCG119650.019.36624344
TCGGCGC127000.018.13215645
ATGCGCG80550.017.73491344
TCACGCT193000.017.7055238
AACGCTA192000.017.09945723
TTCGACG19500.017.0736356
ACGCTAC194400.016.93465224
GTTAGTT155350.016.91423844
GTCCGTT32400.016.5462841
CTCCGAC134100.016.4277691
CGCTACC200200.016.41034925
TACGGTA132950.016.15979844
GCCGGCT229600.015.9815051
GTCGCTA60350.015.88405527
CTACACG71500.015.86680145
CCCGTAT32200.015.66971
CGTACGC147200.015.6499385
AAAACGC213150.015.58902221
Produced by FastQC (version 0.11.5)