FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44754012
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGCTCTTCCCTGTTCACTCGCCGTTACTGAGGGAATCCTGGTTAGTTT703210.15712781236238663No Hit
CTTCCGTACGCCACATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGC501560.11207039940910772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC100850.018.6966024
ACCGCGT102950.018.3555496
CCCGTCT147650.018.0141581
GTTAGTT204000.017.64389844
CAGACCG108750.017.5866933
GACCGCG107950.017.4251965
AACGCTA87300.017.34291523
ACGGTAC113300.016.99607545
ACGCTAC91300.016.68303524
TACGGTA117000.016.42013744
CCGCGTT115900.016.3237977
TACTAAA205450.016.0083839
CTACTAA200300.015.9145968
CGCGTTC120000.015.747258
GAACGAT120600.015.6315345
GTCTCTA173500.015.277084
CGTCTCT150900.015.1346133
CGCTACC101300.014.94816925
CCTTACG130750.014.91745741
TTACGGT131900.014.66783943
Produced by FastQC (version 0.11.5)