FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851308

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851308
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48892621
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGAT1155100.23625241935792315No Hit
GCCAGCTTCACGCTCAGGAGAAAACGCTACCTCTCTTCCTCGTGGTTTTCG545360.11154239409664703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGAT216550.025.2791645
AGACCGC268700.020.4569034
AGAACGA269700.020.38032244
ACCGCGT274450.019.9955166
CAGACCG277600.019.9314613
AACGCTA152850.019.65209223
GACCGCG279500.019.618145
ACGCTAC154750.019.41104524
CCGCGTT286550.019.1506867
CGCGTTC292150.018.7836428
TCACGCT168050.018.7038828
CGCTACC165250.018.12340425
AATACGG304600.018.01703532
ATACGGA314000.017.5559633
TACGGAG314450.017.48074534
AAGAACG317350.017.33440443
TCAGACC326650.017.3321342
AAAACGC175600.017.25993521
GCGTTCT320950.017.1608589
CAATACG324100.016.95418431
Produced by FastQC (version 0.11.5)