FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851309

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851309
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48892621
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG501660.1026044400442349No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTAG181550.013.92796245
GGGAGGT292050.012.8430243
GGCATAG199200.012.68380835
GTTGTCG32550.012.64823726
GGTCACT276600.012.4138627
ATAGGGG205700.012.41314238
GTCACTC284350.012.2734018
GGAGGTC297350.012.0066194
AGGTCAC301900.011.8425056
GAGGTCA310600.011.7042665
GAGCTTA225750.011.34009944
ACTCTCC310450.011.277369511
TGTCGTT38200.011.18857728
TGGCATA231650.011.11099534
CATAGGG243100.010.56886737
GCATAGG252450.010.19537536
TCACTCT357400.010.09872159
TCAGACG88800.010.040132
TGGGAGG284750.09.8358912
CACTCTC360500.09.69915410
Produced by FastQC (version 0.11.5)