FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51330693
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT94450.023.749876
AGACCGC97000.023.2183464
GACCGCG98200.022.842975
TTCGACG28050.021.8976676
CCGCGTT103950.021.536087
CAGACCG108850.020.9387113
CGCGTTC107600.020.8473558
TTCGGCG72250.018.09282544
TCGGCGC72600.017.85064545
CGTACGC76400.017.434015
TCGACGA35800.017.2200987
GAACGAT130150.017.14887245
CCGTACG78050.017.0942754
TATGCCG20600.016.8198345
AATACGG137900.016.33211932
ATGCGCG27100.016.18949744
GTCCGTT39950.015.9606551
ATACGGA143050.015.80705333
CGCGGGG83950.015.75902631
CGACGAG36100.015.7058488
Produced by FastQC (version 0.11.5)