FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851317

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851317
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51330693
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGTCG27750.013.7812126
GGCATAG126000.013.28696135
ATAGGGG133850.012.60902238
ACCCGGC77450.012.374975
GGTCACT210950.011.9880087
AGCTTAG144950.011.78138745
GTCACTC222700.011.608078
CATAGGG147600.011.49505937
ACGCGCG28300.011.20849320
TGGCATA154900.011.0694834
TCAGACG72300.011.0486042
GGGAGGT255850.011.0288313
CGTCGAT21900.010.99205112
TCACCCG96150.010.8823083
GGAGGTC247050.010.7370074
GCATAGG158050.010.72070436
TGCGTCG23650.010.65484410
GAGCTTA160950.010.65283744
AGGTCAC251700.010.5027296
ACTCTCC245500.010.43836511
Produced by FastQC (version 0.11.5)