FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851319

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851319
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44335949
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA549190.12387013527104156No Hit
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG467240.10538626341346613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA10350.014.99548937
GTCACTC239500.013.7305528
GGTCACT244800.013.4592357
GGCATAG180550.013.443768535
AGCTTAG181700.013.30974645
AGGTCAC255200.013.2781846
GGGAGGT278000.013.2558763
ATAGGGG192900.012.74548938
GAGGTCA272700.012.7333025
GTTGTCG30100.012.25700126
ATAGCGT12900.012.20676136
GGAGGTC281900.012.2047364
ACTCTCC276200.012.12663511
TGGCATA204200.012.05197234
GAGCTTA205900.011.83290344
CATAGGG220200.011.15468237
TGCCCGT138800.010.89178344
TCACTCT312600.010.7645179
GCATAGG230050.010.66828636
AACTCTA94650.010.62436945
Produced by FastQC (version 0.11.5)