FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004851321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004851321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48499117
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGAGGTCACTCTCCCCAGGCTCTGTCCAAGTGGCATAGGGGAGCTTAG654380.13492616783105554No Hit
TTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAA567900.11709491535691259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGGTC298950.015.1515934
GGGAGGT319600.014.6446823
AGCTTAG230500.014.2415945
ATAGGGG243550.013.6925538
GAGGTCA338300.013.44222455
GGCATAG246100.013.42269135
GTCACTC335050.013.0546498
GGTCACT338900.012.9530597
CACCGAA234250.012.75535745
ACTCTCC348300.012.64171711
GAGCTTA261750.012.58517944
AGGTCAC372100.012.2516266
GCACCGA244700.012.17472144
TGGCATA285900.011.609220534
CATAGGG291850.011.42651337
TCACTCT395200.011.2783659
CACTCTC388200.011.22649510
TGGGAGG326300.011.0225372
GCATAGG304750.010.93544636
TAGGGGA417550.010.58964739
Produced by FastQC (version 0.11.5)